Electrospray tandem mass spectrometry of 2H-chromenes

Electrospray tandem mass spectrometry of 2H-chromenes.

Several 2H-chromenes derived from carbazoles were analyzed by electrospray tandem mass spectrometry. The 2H-chromenes constitute an important class of compounds that exhibit photochromic activity. The fragmentation pathways of the protonated molecular species [M+H]+ were studied, and main fragmentation pathways of these compounds were identified. Fragmentation pathways of [M+D]+ ions were also ...

Electrospray and tandem mass spectrometry in biochemistry.

Over the last 20 years, biological MS has changed out of all recognition. This is primarily due to the development in the 1980s of 'soft ionization' methods that permit the ionization and vaporization of large, polar, and thermally labile biomolecules. These developments in ionization mode have driven the design and manufacture of smaller and cheaper mass analysers, making the mass spectrometer...

chromatography–electrospray ionization tandem mass spectrometry

Electrospray tandem mass spectrometry of intact beta-chain hemoglobin variants.

In this report, we present data to illustrate how human hemoglobin (Hb) variants can be identified by electrospray tandem mass spectrometry (MS/MS) of the intact Hb chains following the one-step dilution of whole blood. MS/MS spectra were recorded on a series of intact beta-chain human Hb variants. The resultant spectra were interpreted, and using the information gleaned from the fragmentation ...

Methanol-associated matrix effects in electrospray ionization tandem mass spectrometry.

BACKGROUND Matrix effects can profoundly reduce the performance of electrospray ionization mass spectrometry. Preliminary observations indicated that the methanol used in the mobile phase could be a source of differential ionization or ion suppression. METHODS Drug stability studies, analysis of biological extracts, mixing experiments, and postcolumn infusions were used to test 9 commercial m...